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Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium.

ABSTRACT: Bacteria are key denitrifiers in the reduction of nitrate (NO₃ ^--N), which is a contaminant in wastewater treatment plants (WWTPs). They can also produce carbon dioxide (CO₂) and nitrous oxide (N₂O). In this study, the autotrophic hydrogen-oxidizing bacterium Rhodoblastus sp. TH20 was isolated for sustainable treatment of NO₃ ^--N in wastewater. Efficient removal of NO₃ ^--N and recovery of biomass nitrogen were achieved. Up to 99% of NO₃ ^--N was removed without accumulation of nitrite and N₂O, consuming CO₂ of 3.25 mol for each mole of NO₃ ^--N removed. The overall removal rate of NO₃ ^--N reached 1.1 mg L^-1 h^-1 with a biomass content of approximately 0.71 g L^-1 within 72 h. TH20 participated in NO₃ ^--N assimilation and aerobic denitrification. Results from ¹⁵N-labeled-nitrate test indicated that removed NO₃ ^--N was assimilated into organic nitrogen, showing an assimilation efficiency of 58%. Seventeen amino acids were detected, accounting for 43% of the biomass. Nitrogen loss through aerobic denitrification was only approximately 42% of total nitrogen. This study suggests that TH20 can be applied in WWTP facilities for water purification and production of valuable biomass to mitigate CO₂ and N₂O emissions.

SUBMITTER: Chen YZ

PROVIDER: S-EPMC9487994 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium.

Chen Yi-Zhen YZ Zhang Li-Juan LJ Ding Ling-Yun LY Zhang Yao-Yu YY Wang Xi-Song XS Qiao Xue-Jiao XJ Pan Bao-Zhu BZ Wang Zhi-Wu ZW Xu Nan N Tao Hu-Chun HC

Environmental science and ecotechnology 20220123

Bacteria are key denitrifiers in the reduction of nitrate (NO3 --N), which is a contaminant in wastewater treatment plants (WWTPs). They can also produce carbon dioxide (CO2) and nitrous oxide (N2O). In this study, the autotrophic hydrogen-oxidizing bacterium Rhodoblastus sp. TH20 was isolated for sustainable treatment of NO3 --N in wastewater. Efficient removal of NO3 --N and recovery of biomas ...[more]

PMID: 36157854

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Project description:Nitrate-reducing Fe(II)-oxidizing microorganisms were described for the first time ca. 20 years ago. Most pure cultures of nitrate-reducing Fe(II) oxidizers can oxidize Fe(II) only under mixotrophic conditions, i.e., when an organic cosubstrate is provided. A small number of nitrate-reducing Fe(II)-oxidizing cultures have been proposed to grow autotrophically, but unambiguous evidence for autotrophy has not always been provided. Thus, it is still unclear whether or to what extent Fe(II) oxidation coupled to nitrate reduction is an enzymatically catalyzed and energy-yielding autotrophic process or whether Fe(II) is abiotically oxidized by nitrite from heterotrophic nitrate reduction. The aim of the present study was to find evidence for the existence of autotrophic nitrate-reducing Fe(II) oxidizers in coastal marine sediments. Microcosm incubations showed that with increasing incubation times, the stoichiometric ratio of reduced nitrate/oxidized Fe(II) [NO3-reduced/Fe(II)oxidized] decreased, indicating a decreasing contribution of heterotrophic denitrification and/or an increasing contribution of autotrophic nitrate-reducing Fe(II) oxidation over time. After incubations of sediment slurries for >10 weeks, nitrate-reducing activity ceased, although nitrate was still present. This suggests that heterotrophic nitrate reduction had ceased due to the depletion of readily available organic carbon. However, after the addition of Fe(II) to these batch incubation mixtures, the nitrate-reducing activity resumed, and Fe(II) was oxidized, indicating the activity of autotrophic nitrate-reducing Fe(II) oxidizers. The concurrent reduction of 14C-labeled bicarbonate concentrations unambiguously proved that autotrophic C fixation occurred during Fe(II) oxidation and nitrate reduction. Our results clearly demonstrated that autotrophic nitrate-reducing Fe(II)-oxidizing bacteria were present in the investigated coastal marine sediments.ImportanceTwenty years after the discovery of nitrate-reducing Fe(II) oxidizers, it is still controversially discussed whether autotrophic nitrate-reducing Fe(II)-oxidizing microorganisms exist and to what extent Fe(II) oxidation in this reduction/oxidation process is enzymatically catalyzed or which role abiotic side reactions of Fe(II) with reactive N species play. Most pure cultures of nitrate-reducing Fe(II) oxidizers are mixotrophic; i.e., they need an organic cosubstrate to maintain their activity over several cultural transfers. For the few existing autotrophic isolates and enrichment cultures, either the mechanism of nitrate-reducing Fe(II) oxidation is not known or evidence for their autotrophic lifestyle is controversial. In the present study, we provide evidence for the existence of autotrophic nitrate-reducing Fe(II) oxidizers in coastal marine sediments. The evidence is based on stoichiometries of nitrate reduction and Fe(II) oxidation determined in microcosm incubations and the incorporation of carbon from CO2 under conditions that favor the activity of nitrate-reducing Fe(II) oxidizers.

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Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium.

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Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium.

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