Standardized high-throughput biomonitoring using DNA metabarcoding: Strategies for the adoption of automated liquid handlers.

ABSTRACT: Reliable and comprehensive monitoring data are required to trace and counteract biodiversity loss. High-throughput metabarcoding using DNA extracted from community samples (bulk) or from water or sediment (environmental DNA) has revolutionized biomonitoring, given the capability to assess biodiversity across the tree of life rapidly with feasible effort and at a modest price. DNA metabarcoding can be upscaled to process hundreds of samples in parallel. However, while automated high-throughput analysis workflows are well-established in the medical sector, manual sample processing still predominates in biomonitoring laboratory workflows limiting the upscaling and standardization for routine monitoring applications. Here we present an automated, scalable, and reproducible metabarcoding workfl

SUBMITTER: Buchner D 

PROVIDER: S-EPMC9488008 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature