Transposon Insertion Sequencing, a Global Measure of Gene Function.

ABSTRACT: The goal of genomics and systems biology is to understand how complex systems of factors assemble into pathways and structures that combine to form living organisms. Great advances in understanding biological processes result from determining the function of individual genes, a process that has classically relied on characterizing single mutations. Advances in DNA sequencing has made available the complete set of genetic instructions for an astonishing and growing number of species. To understand the function of this ever-increasing number of genes, a high-throughput method was developed that in a single experiment can measure the function of genes across the genome of an organism. This occurred approximately 10 years ago, when high-throughput DNA sequencing was combined with advances in t

SUBMITTER: van Opijnen T 

PROVIDER: S-EPMC9488914 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature