A real-time measurement system for gene expression rhythms from deep tissues of freely moving mice under light-dark conditions.

ABSTRACT: Clock gene expression in most organs of the living body exhibits a diurnal rhythm synchronized with the external 24 h light-dark (LD) cycle via circadian pacemaker suprachiasmatic nucleus (SCN). Disturbances in clock gene expression due to desynchronization of clock gene expression of the external LD cycle are risk factors for developing various diseases. Measuring the in vivo clock genes expression rhythm for a long duration under LD conditions can greatly contribute to understand the pathogenic mechanism of the disease caused by the disturbance of the biological rhythm. However, it is presently difficult to continuously measure gene expression for a long duration under LD conditions. In present study, we succeeded in measuring Period1 (Per1) gene expression under LD

SUBMITTER: Nakaya M 

PROVIDER: S-EPMC9489493 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature