Realistic biomarkers from plasma extracellular vesicles for detection of beryllium exposure.

ABSTRACT:

Purpose

Exposures related to beryllium (Be) are an enduring concern among workers in the nuclear weapons and other high-tech industries, calling for regular and rigorous biological monitoring. Conventional biomonitoring of Be in urine is not informative of cumulative exposure nor health outcomes. Biomarkers of exposure to Be based on non-invasive biomonitoring could help refine disease risk assessment. In a cohort of workers with Be exposure, we employed blood plasma extracellular vesicles (EVs) to discover novel biomarkers of exposure to Be.

Methods

EVs were isolated from plasma using size-exclusion chromatography and subjected to mass spectrometry-based proteomics. A protein-based classifier was developed using LASSO regression and validated by ELISA.

Results

We dis

SUBMITTER: Adduri RSR 

PROVIDER: S-EPMC9489591 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature