Screening microbially produced Δ⁹-tetrahydrocannabinol using a yeast biosensor workflow.

ABSTRACT: Microbial production of cannabinoids promises to provide a consistent, cheaper, and more sustainable supply of these important therapeutic molecules. However, scaling production to compete with traditional plant-based sources is challenging. Our ability to make strain variants greatly exceeds our capacity to screen and identify high producers, creating a bottleneck in metabolic engineering efforts. Here, we present a yeast-based biosensor for detecting microbially produced Δ⁹-tetrahydrocannabinol (THC) to increase throughput and lower the cost of screening. We port five human cannabinoid G protein-coupled receptors (GPCRs) into yeast, showing the cannabinoid type 2 receptor, CB2R, can couple to the yeast pheromone response pathway and report on the concentration of a variety of

SUBMITTER: Shaw WM 

PROVIDER: S-EPMC9489785 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature