Hepatic kinome atlas: An in-depth identification of kinase pathways in liver fibrosis of humans and rodents.

ABSTRACT:

Background and aims

Resolution of pathways that converge to induce deleterious effects in hepatic diseases, such as in the later stages, have potential antifibrotic effects that may improve outcomes. We aimed to explore whether humans and rodents display similar fibrotic signaling networks.

Approach and results

We assiduously mapped kinase pathways using 340 substrate targets, upstream bioinformatic analysis of kinase pathways, and over 2000 random sampling iterations using the PamGene PamStation kinome microarray chip technology. Using this technology, we characterized a large number of kinases with altered activity in liver fibrosis of both species. Gene expression and immunostaining analyses validated many of these kinases as bona fide signaling events. Surprisingly, the

SUBMITTER: Creeden JF 

PROVIDER: S-EPMC9489820 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature