A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen.

ABSTRACT:

Aims

The well-documented relationship between sperm oxidation and male infertility strongly encourages the development of assays for reactive oxygen species detection in semen samples. The present study aims to apply the microplate-based 2',7'-dichlorofluorescein diacetate assay to the evaluation of oxidative stress in unprocessed whole semen, thus avoiding sample centrifugations and other manipulations that may cause significant reactive oxygen species increments.

Main methods

The fluorescence assay consisted in the quantification of both intracellular and extracellular reactive oxygen species levels in unwashed semen specimens by using the probe 2',7'-dichlorofluorescein diacetate into a 96-well plate. The method was useful for the preliminary assessment of the oxidation l

SUBMITTER: Benedetti S 

PROVIDER: S-EPMC9489972 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature