Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides.

ABSTRACT: FlAlyA, a PL7 alginate lyase with industrial potential, is widely applied in the preparation the alginate oligosaccharide because of its high activity of degradation the alginate. However, heat inactivation still limits the industrial application of FlAlyA. To further enhance its thermostability, a group of mutants were designed, according to evaluating the B-factor value and free energy change via computer-aided calculation. 25 single-point mutants and one double-points mutant were carried out by site-directed mutagenesis. The optimal two single-point mutants H176D and H71K showed 1.20 and 0.3°C increases in the values of T _m, while 7.58 and 1.73 min increases in the values of half-life (t _1/2) at 50°C, respectively, compared with that of the wild-type

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9490058 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature