Impact of preservation method and storage period on ribosomal metabarcoding of marine microbes: Implications for remote automated samplings.

ABSTRACT: Automated sampling technologies can enhance the temporal and spatial resolution of marine microbial observations, particularly in remote and inaccessible areas. A critical aspect of automated microbiome sampling is the preservation of nucleic acids over long-term autosampler deployments. Understanding the impact of preservation method on microbial metabarcoding is essential for implementing genomic observatories into existing infrastructure, and for establishing best practices for the regional and global synthesis of data. The present study evaluates the effect of two preservatives commonly used in autosampler deployments (mercuric chloride and formalin) and two extraction kits (PowerWater and NucleoSpin) on amplicon sequencing of 16S and 18S rRNA gene over 50 weeks of sample storage. Our

SUBMITTER: Wietz M 

PROVIDER: S-EPMC9490091 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature