A comparative study of three chemometrics methods combined with excitation–emission matrix fluorescence for quantification of the bioactive compounds aesculin and aesculetin in Cortex Fraxini

ABSTRACT: Cortex Fraxini is an important traditional Chinese herbal medicine with various medical functions. Aesculin and aesculetin are the main effective components of Cortex Fraxini. The fluorescence signals of the two compounds have a high degree of overlap with each other, making quantitative analysis difficult with conventional analytical methods. In the present study, different chemometrics methods, including lasso regression (LAR), interval partial least squares (iPLS), and multidimensional partial least squares (N-PLS) methods, were employed and combined with excitation–emission matrix (EEM) fluorescence for the purpose of accurate quantification of aesculin and aesculetin in Cortex Fraxini samples. The most satisfactory results were obtained by using the N-PLS method based on the EEM spect

SUBMITTER: Li Z 

PROVIDER: S-EPMC9490370 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature