Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses.

ABSTRACT: X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights into structural dynamics of biological macromolecule solutions. However, very high dose rates can lead to beam-induced dynamics and structural changes due to radiation damage. Here, we probe the dynamics of dense antibody protein (Ig-PEG) solutions using megahertz X-ray photon correlation spectroscopy (MHz-XPCS) at the European XFEL. By varying the total dose and dose rate, we identify a regime for measuring the motion of proteins in their first coordination shell, quantify XFEL-induced effects such as driven motion, and map out the extent of agglomeration dynamics. The results indicate that for average dose rates below 1.06 kGy μs^-1 in a time window up to 10 μs, it is possible to captur

SUBMITTER: Reiser M 

PROVIDER: S-EPMC9490738 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature