Enterococcus faecalis rnc gene modulates its susceptibility to disinfection agents: a novel approach against biofilm.

ABSTRACT:

Background

Enterococcus faecalis (E. faecalis) plays an important role in the failure of root canal treatment and refractory periapical periodontitis. As an important virulence factor of E. faecalis, extracellular polysaccharide (EPS) serves as a matrix to wrap bacteria and form biofilms. The homologous rnc gene, encoding Ribonuclease III, has been reported as a regulator of EPS synthesis. In order to develop novel anti-biofilm targets, we investigated the effects of the rnc gene on the biological characteristics of E. faecalis, and compared the biofilm tolerance towards the typical root canal irrigation agents and traditional Chinese medicine fluid Pudilan.

Methods

E. faecalis rnc gene overexpression (rnc+) and low-expression (rnc-) strains were constructed. The growth curv

SUBMITTER: Xia M 

PROVIDER: S-EPMC9490916 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature