Genomics and epigenetics guided identification of tissue-specific genomic safe harbors.

ABSTRACT:

Background

Genomic safe harbors are regions of the genome that can maintain transgene expression without disrupting the function of host cells. Genomic safe harbors play an increasingly important role in improving the efficiency and safety of genome engineering. However, limited safe harbors have been identified.

Results

Here, we develop a framework to facilitate searches for genomic safe harbors by integrating information from polymorphic mobile element insertions that naturally occur in human populations, epigenomic signatures, and 3D chromatin organization. By applying our framework to polymorphic mobile element insertions identified in the 1000 Genomes project and the Genotype-Tissue Expression (GTEx) project, we identify 19 candidate safe harbors in blood cells and 5 in

SUBMITTER: Shrestha D 

PROVIDER: S-EPMC9490961 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature