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The N-terminal substrate specificity of the SurE peptide cyclase.

ABSTRACT: SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue.

SUBMITTER: Fazal A

PROVIDER: S-EPMC9491159 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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The N-terminal substrate specificity of the SurE peptide cyclase.

Fazal Asif A Wheeler Jake J Webb Michael E ME Seipke Ryan F RF

Organic & biomolecular chemistry 20220921 36

SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides <i>in vivo</i>, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue. ...[more]

PMID: 36062889

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