Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes.

ABSTRACT: Detection of nucleic acid amplification has typically required sophisticated laboratory instrumentation, but as the amplification techniques have moved away from the lab, complementary detection techniques have been implemented to facilitate point-of-care, field, and even at-home applications. Simple visual detection approaches have been widely used for isothermal amplification methods, but have generally displayed weak color changes or been highly sensitive to sample and atmospheric effects. Here we describe the use of pyridylazophenol dyes and binding to manganese ion to produce a strong visible color that changes in response to nucleic acid amplification. This detection approach is easily quantitated with absorbance, rapidly and clearly visible by eye, robust to sample effects, and nota

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9491268 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature