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RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction.

ABSTRACT: Polyadenylate [poly(A)] tail addition to the 3' end of a wide range of RNAs is a highly conserved modification that plays a central role in cellular RNA function. Elements for nuclear expression (ENEs) are cis-acting RNA elements that stabilize poly(A) tails by sequestering them in RNA triplex structures. A crystal structure of a double ENE from a rice hAT transposon messenger RNA complexed with poly(A)28 at a resolution of 2.89 angstroms reveals multiple modes of interaction with poly(A), including major-groove triple helices, extended minor-groove interactions with RNA double helices, a quintuple-base motif that transitions poly(A) from minor-groove associations to major-groove triple helices, and a poly(A) 3'-end binding pocket. Our findings both expand the repertoire of motifs involved in long-range RNA interactions and provide insights into how polyadenylation can protect an RNA's extreme 3' end.

SUBMITTER: Torabi SF 

PROVIDER: S-EPMC9491362 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction.

Torabi Seyed-Fakhreddin SF   Vaidya Anand T AT   Tycowski Kazimierz T KT   DeGregorio Suzanne J SJ   Wang Jimin J   Shu Mei-Di MD   Steitz Thomas A TA   Steitz Joan A JA  

Science (New York, N.Y.) 20210107 6529

Polyadenylate [poly(A)] tail addition to the 3' end of a wide range of RNAs is a highly conserved modification that plays a central role in cellular RNA function. Elements for nuclear expression (ENEs) are cis-acting RNA elements that stabilize poly(A) tails by sequestering them in RNA triplex structures. A crystal structure of a double ENE from a rice hAT transposon messenger RNA complexed with poly(A)<sub>28</sub> at a resolution of 2.89 angstroms reveals multiple modes of interaction with pol  ...[more]

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