Characterization of cellular senescence in aging skeletal muscle.

ABSTRACT: Senescence is a cell fate that contributes to multiple aging-related pathologies. Despite profound age-associated changes in skeletal muscle (SkM), whether its constituent cells are prone to senesce has not been methodically examined. Herein, using single cell and bulk RNA-sequencing and complementary imaging methods on SkM of young and old mice, we demonstrate that a subpopulation of old fibroadipogenic progenitors highly expresses p16 ^Ink4a together with multiple senescence-related genes and, concomitantly, exhibits DNA damage and chromatin reorganization. Through analysis of isolated myofibers, we also detail a senescence phenotype within a subset of old cells, governed instead by p2 ^Cip1 . Administration of a senotherapeutic interventio

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9491365 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature