Axin2-lineage cells contribute to neonatal tendon regeneration.

ABSTRACT:

Purpose

Tendon injuries are a challenging clinical problem with few treatment options. Identifying the molecular regulators of tendon is required for the development of new therapies. While the Wnt pathway is critical for the maintenance and differentiation of many tissues, the role of Wnt signaling in tendon cell biology remains largely unexplored.

Methods

The effects of Wnt activation were tested in vitro using neonatal tendon-derived cells cultured in 2D and 3D conditions. The inducible Axin2CreERT2 was then used to label Axin2+ cells in vivo and cells were traced during neonatal tendon regeneration.

Results

We showed that activation of Wnt signaling results in proliferation of neonatal tendon cells. While tendon marker expression was inhibited by Wnt

SUBMITTER: Walia B 

PROVIDER: S-EPMC9491382 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature