LsNRL4 enhances photosynthesis and decreases leaf angles in lettuce.

ABSTRACT: Lettuce (Lactuca sativa) is one of the most important vegetables worldwide and an ideal plant for producing protein drugs. Both well-functioning chloroplasts that perform robust photosynthesis and small leaf angles that enable dense planting are essential for high yields. In this study, we used an F₂ population derived from a cross between a lettuce cultivar with pale-green leaves and large leaf angles to a cultivar with dark-green leaves and small leaf angles to clone LsNRL4, which encodes an NPH3/RPT2-Like (NRL) protein. Unlike other NRL proteins in lettuce, the LsNRL4 lacks the BTB domain. Knockout mutants engineered using CRISPR/Cas9 and transgenic lines overexpressing LsNRL4 verified that LsNRL4 contributes to chloroplast development, photosynthesis and leaf angle. The LsNR

SUBMITTER: An G 

PROVIDER: S-EPMC9491448 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature