The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy.

ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy can be used to determine the structure, dynamics and interactions of proteins. However, protein NMR requires stable isotope labelling for signal detection. The cells used for the production of recombinant proteins must therefore be grown in medium containing isotopically labelled substrates. Stable isotope labelling is well established in Escherichia coli, but bacteria are only suitable for the production of simple proteins without post-translational modifications. More complex proteins require eukaryotic production hosts, but their growth can be impaired by labelled media, thus reducing product yields and increasing costs. To address this limitation, we used media supplemented with isotope-labelled substrates to cultivate the tobacco-derived c

SUBMITTER: Opdensteinen P 

PROVIDER: S-EPMC9491462 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature