Visual closed dumbbell-mediated isothermal amplification (CDA) for on-site detection of Rickettsia raoultii.

ABSTRACT: Spotted fever group (SFG) rickettsioses are important zoonoses, threatening human health seriously and gradually attracting more attention in the world. SFG rickettsiae are classified as neglected pathogens. If these pathogens are detected at all, they are usually recognized very late in the infection through indirect detection of specific antibodies. Previous studies have shown that Rickettsia raoultii (R. raoultii), a member of the SFG rickettsiae, occurs with increasing incidence in remote countries. Therefore, a rapid detection method for R. raoultii is in urgently need. In this study, a R. raoultii diagnosis method by closed dumbbell-mediated isothermal amplification (R-CDA) assay targeting a conserved sequence of the outer membrane protein A (OmpA) gene with high sensitivity and spec

SUBMITTER: Gui Z 

PROVIDER: S-EPMC9491570 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature