Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans.

ABSTRACT: Extracellular vesicles (EVs) may mediate intercellular communication by carrying protein and RNA cargo. The composition, biology, and roles of EVs in physiology and pathology have been primarily studied in the context of biofluids and in cultured mammalian cells. The experimental tractability of C. elegans makes for a powerful in vivo animal system to identify and study EV cargo from its cellular source. We developed an innovative method to label, track, and profile EVs using genetically encoded, fluorescent-tagged EV cargo and conducted a large-scale isolation and proteomic profiling. Nucleic acid binding proteins (∼200) are overrepresented in our dataset. By integrating our EV proteomic dataset with single-cell transcriptomic data, we identified and validated ciliary EV cargo: CD9-like t

SUBMITTER: Nikonorova IA 

PROVIDER: S-EPMC9491618 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature