Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.

ABSTRACT: Upon internalization, many surface membrane proteins are recycled back to the plasma membrane. Although these endosomal trafficking pathways control surface protein activity, the precise regulatory features and division of labor between interconnected pathways are poorly defined. In yeast, we show recycling back to the surface occurs through distinct pathways. In addition to retrograde recycling pathways via the late Golgi, used by synaptobrevins and driven by cargo ubiquitination, we find nutrient transporter recycling bypasses the Golgi in a pathway driven by cargo deubiquitination. Nutrient transporters rapidly internalize to, and recycle from, endosomes marked by the ESCRT-III associated factor Ist1. This compartment serves as both "early" and "recycling" endosome. We show Ist1 is ubiq

SUBMITTER: Laidlaw KME 

PROVIDER: S-EPMC9491851 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature