Chemokine Assay Matrix Defines the Potency of Human Bone Marrow Mesenchymal Stromal Cells.

ABSTRACT: Potency analysis of mesenchymal stromal cells (MSCs) is required for their use in advanced clinical trials. Assay matrix strategy evaluating more than a single property of MSCs is an emerging strategy in potency analysis. Here we developed an assay matrix approach focusing on the secretory chemokine responses of MSCs using multiplex analytical method. MSCs' innate fitness in secreting matrix of chemokines is correlated with their metabolic fitness in differential degrees. In addition, innately secreting chemokines are correlated among themselves in a unique pattern. MSC's matrix chemokine responses to exogenous stimulation of IFNγ and/or TNFα are distinct. However, the combination of IFNγ and TNFα is superior than individual stimulations in eliciting robust and broad matrix chemokine respo

SUBMITTER: Lipat AJ 

PROVIDER: S-EPMC9492268 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature