Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics.

ABSTRACT:

Objective

To explore the possible mechanism of fluid shear stress on human nucleus pulposus cells based on label-free proteomics technology.

Methods

The human nucleus pulposus cell line was purchased and subcultured in vitro. The Flexcell STR-4000 multiflow field cell fluid shear stress loading culture system was used to apply continuous laminar fluid shear stress (12 dyne/cm², 45 mins) to the monolayer adherent cells. Those without mechanical loading were used as the control group, and those subjected to fluid shear loading were used as the experimental group. Differential protein expression was identified using mass spectrometry identification technology, and bioinformatics analysis was performed using Gene Ontology GO (Gene Ontology) and Kyoto Encyclopedia of G

SUBMITTER: Xie LY 

PROVIDER: S-EPMC9492342 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature