Macrophages in obesity are characterised by increased IL-1β response to calcium-sensing receptor signals.

ABSTRACT:

Objective

Obesity is complicated by inflammatory activation of the innate immune system. Stimulation of the calcium-sensing receptor (CaSR) by extra-cellular calcium ions ([Ca²⁺]_ex) can trigger NLRP3 inflammasome activation and inflammation. We hypothesised, that this mechanism might contribute to the activation of adipose tissue (AT) in obesity, and investigated [Ca²⁺]_ex-induced, CaSR mediated IL-1β release by macrophages in obesity.

Methods

[Ca²⁺]_ex-induced IL-1β release was investigated in monocyte-derived macrophages (MDM) generated from peripheral blood of patients with obesity and from normal-weight controls. Visceral and subcutaneous AT biosamples were stimulated with [Ca²⁺]_ex, and

SUBMITTER: Thrum S 

PROVIDER: S-EPMC9492543 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature