Novel method for detecting complement C3 deposition on Staphylococcus aureus.

ABSTRACT: The primary host response to Staphylococcus aureus infection occurs via complement. Complement is an elegant evolutionarily conserved system, playing essential roles in early defences by working in concert with immune cells to survey, label and destroy microbial intruders and coordinate inflammation. Currently the exact mechanisms employed by S. aureus to manipulate and evade complement is not clear and is hindered by the lack of accurate molecular tools that can report on complement deposition on the bacterial surface. Current gold-standard detection methods employ labelled complement-specific antibodies and flow cytometry to determine complement deposited on bacteria. These methods are restricted by virtue of the expression of the S. aureus immunoglobulin binding proteins, Protein A and

SUBMITTER: Wonfor T 

PROVIDER: S-EPMC9492775 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature