Reaction-based fluorogenic probes for detecting protein cysteine oxidation in living cells.

ABSTRACT: 'Turn-on' fluorescence probes for detecting H₂O₂ in cells are established, but equivalent tools to monitor the products of its reaction with protein cysteines have not been reported. Here we describe fluorogenic probes for detecting sulfenic acid, a redox modification inextricably linked to H₂O₂ signaling and oxidative stress. The reagents exhibit excellent cell permeability, rapid reactivity, and high selectivity with minimal cytotoxicity. We develop a high-throughput assay for measuring S-sulfenation in cells and use it to screen a curated kinase inhibitor library. We reveal a positive association between S-sulfenation and inhibition of TK, AGC, and CMGC kinase group members including GSK3, a promising target for neurological disorders. Proteom

SUBMITTER: Ferreira RB 

PROVIDER: S-EPMC9492777 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature