Quantitative interactome proteomics identifies a proteostasis network for GABA_A receptors.

ABSTRACT: Gamma-aminobutyric acid type A (GABA_A) receptors are the primary inhibitory neurotransmitter-gated ion channels in the mammalian central nervous system. Maintenance of GABA_A receptor protein homeostasis (proteostasis) in cells utilizing its interacting proteins is essential for the function of GABA_A receptors. However, how the proteostasis network orchestrates GABA_A receptor biogenesis in the endoplasmic reticulum is not well understood. Here, we employed a proteomics-based approach to systematically identify the interactomes of GABA_A receptors. We carried out a quantitative immunoprecipitation-tandem mass spectrometry analysis utilizing stable isotope labeling by amino acids in cell culture. Furthermore, we performed comparative prote

SUBMITTER: Wang YJ 

PROVIDER: S-EPMC9493394 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature