A Direct Infusion Probe for Rapid Metabolomics of Low-Volume Samples.

ABSTRACT: Targeted and nontargeted metabolomics has the potential to evaluate and detect global metabolite changes in biological systems. Direct infusion mass spectrometric analysis enables detection of all ionizable small molecules, thus simultaneously providing information on both metabolites and lipids in chemically complex samples. However, to unravel the heterogeneity of the metabolic status of cells in culture and tissue a low number of cells per sample should be analyzed with high sensitivity, which requires low sample volumes. Here, we present the design and characterization of the direct infusion probe, DIP. The DIP is simple to build and position directly in front of a mass spectrometer for rapid metabolomics of chemically complex biological samples using pneumatically assisted electrospra

SUBMITTER: Marques C 

PROVIDER: S-EPMC9494293 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature