Acoustic Focusing of Protein Crystals for In-Line Monitoring and Up-Concentration during Serial Crystallography.

ABSTRACT: Serial femtosecond crystallography (SFX) has become one of the standard techniques at X-ray free-electron lasers (XFELs) to obtain high-resolution structural information from microcrystals of proteins. Nevertheless, reliable sample delivery is still often limiting data collection, as microcrystals can clog both field- and flow-focusing nozzles despite in-line filters. In this study, we developed acoustic 2D focusing of protein microcrystals in capillaries that enables real-time online characterization of crystal size and shape in the sample delivery line after the in-line filter. We used a piezoelectric actuator to create a standing wave perpendicular to the crystal flow, which focused lysozyme microcrystals into a single line inside a silica capillary so that they can be imaged using a hi

SUBMITTER: Hammarstrom B 

PROVIDER: S-EPMC9494305 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature