Comparison of single‑nucleus and single‑cell transcriptomes in hepatocellular carcinoma tissue.

ABSTRACT: Single‑nucleus RNA sequencing (snRNA‑seq) is a method used to analyze gene expression in cells for which isolation is complex, such as those in hepatocellular carcinoma (HCC) tissues. It constitutes an alternative to single‑cell RNA sequencing (scRNA‑seq) by analyzing the nucleus rather than the whole cell; however, whether it can completely replace scRNA‑seq in HCC remains to be clarified. In the present study, scRNA‑seq was compared with snRNA‑seq in tumor tissue obtained from patients with HCC, using the 10X Genomics Chromium platform. Seurat was also used to process the data and compare the differences between the two sequencing methods in identifying different cell types. In the present study, the transcriptomes of 14,349 single nuclei and 9,504 single cells were obtained from the afo

SUBMITTER: Wen F 

PROVIDER: S-EPMC9494604 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature