An Electrical Stimulation Culture System for Daily Maintenance-Free Muscle Tissue Production.

ABSTRACT: Low-labor production of tissue-engineered muscles (TEMs) is one of the key technologies to realize the practical use of muscle-actuated devices. This study developed and then demonstrated the daily maintenance-free culture system equipped with both electrical stimulation and medium replacement functions. To avoid ethical issues, immortal myoblast cells C2C12 were used. The system consisting of gel culture molds, a medium replacement unit, and an electrical stimulation unit could produce 12 TEMs at one time. The contractile forces of the TEMs were measured with a newly developed microforce measurement system. Even the TEMs cultured without electrical stimulation generated forces of almost 2 mN and were shortened by 10% in tetanic contractions. Regarding the contractile forces, electrical st

SUBMITTER: Akiyama Y 

PROVIDER: S-EPMC9494718 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature