Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes.

ABSTRACT: Contractile dysfunction and Ca²⁺ transients are often analyzed at the cellular level as part of a comprehensive assessment of cardiac-induced injury and/or remodeling. One approach for assessing these functional alterations utilizes unloaded shortening and Ca²⁺ transient analyses in primary adult cardiac myocytes. For this approach, adult myocytes are isolated by collagenase digestion, made Ca²⁺ tolerant, and then adhered to laminin-coated coverslips, followed by electrical pacing in serum-free media. The general protocol utilizes adult rat cardiac myocytes but can be readily adjusted for primary myocytes from other species. Functional alterations in myocytes from injured hearts can be compared to sham myocytes and/or to in vitro therapeutic treatments. The

SUBMITTER: Lavey EA 

PROVIDER: S-EPMC9494753 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature