Effects exerted by transcriptional regulator PcaU from Acinetobacter sp. strain ADP1.

ABSTRACT: Protocatechuate degradation is accomplished in a multistep inducible catabolic pathway in Acinetobacter sp. strain ADP1. The induction is brought about by the transcriptional regulator PcaU in concert with the inducer protocatechuate. PcaU, a member of the new IclR family of transcriptional regulators, was shown to play a role in the activation of transcription at the promoter for the structural pca genes, leaving open the participation of additional activators. In this work we show that there is no PcaU-independent transcriptional activation at the pca gene promoter. The minimal inducer concentration leading to an induction response is 10(-5) M protocatechuate. The extent of expression of the pca genes was observed to depend on the nature of the inducing carbon source, and this is assumed

SUBMITTER: Trautwein G 

PROVIDER: S-EPMC94953 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature