Two complementary approaches for efficient isolation of Sertoli cells for transcriptomic analysis.

ABSTRACT: Sertoli cells (SCs) are the only somatic cells that reside in seminiferous tubules of testis. They directly interact with and support the development of germ cells, thus have an indispensable role in the process of spermatogenesis. SCs first appear in a proliferative state and then, with the initiation of the first wave of spermatogenesis, progress to a mature "nurturing" state which supports lifelong continuous sperm production. During this development, the SC transcriptome must adapt rapidly as obstacles in SC maturation often result in deficiencies in male fertility. Due to its importance in spermatogenesis, a reliable, rapid, and precise method for the isolation of high purity, viable and unadulterated SC has been largely missing. We have developed an improved method for the preparatio

SUBMITTER: Petrusova J 

PROVIDER: S-EPMC9495933 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature