Differential Protein Content between Fresh and Freeze-Dried Plasma Rich in Growth Factors Eye Drops.

ABSTRACT: The purpose of this study was to analyze the proteomic composition of plasma rich in growth factors eye drops (PRGF) in comparison to lyophilized PRGF eye drops (PRGF lyo). The differential protein expression of keratocyte (HK) cells after PRGF or PRGF lyo treatment was also determined. Blood from different donors was collected and processed to obtain PRGF and PRGF lyo eye drops. Then, HK cells were treated with both formulations. A proteomic analysis was performed to evaluate the differential proteomic profile between PRGF and PRGF lyo, and the differential protein expression by HK cells after treatment with both blood-derived products. About 280 proteins were detected between both blood-derived formulation, with only 8 of them reaching significant differences. Furthermore, 101 out of 321

SUBMITTER: Anitua E 

PROVIDER: S-EPMC9496081 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature