Dataset Information

Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface.

ABSTRACT: This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells' differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsiveness when exposed to each of the two rmBMP-7 concentrations, irrespective of the surface. Even with 40 ng/mL rmBMP-7, important osteogenic effects were noticed for Control-Ti in terms of cell proliferation potential; Runx2, Osx, Alp, Bsp, Opn, and Smad1 mRNA expression; and in situ ALP activity. For Nano-Ti, the effects were limited to higher Alp, Bsp, and Opn mRNA expression and in situ ALP activity. On both surfaces, the osteogenic potential of UMR-106 cultures remained unaltered with 40 ng/mL rmBMP-7, but it was significantly reduced when the cultures were exposed to the 200 ng/mL concentration. The availability of rmBMP-7 to pre-osteoblastic cells at the concentrations used alters the expression profile of osteoblast markers, indicative of the acquisition of a more advanced stage of osteoblastic differentiation. This occurs less pronouncedly on the nanotextured Ti and without reflecting in higher mineralized matrix production by differentiated osteoblasts on both surfaces.

SUBMITTER: Zuardi LR

PROVIDER: S-EPMC9496167 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface.

Zuardi Leonardo Raphael LR de Oliveira Fabíola Singaretti FS Fernandes Roger Rodrigo RR Gomes Maria Paula Oliveira MPO Spriano Silvia S Nanci Antonio A de Oliveira Paulo Tambasco PT

Biomimetics (Basel, Switzerland) 20220916 3

This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells' differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsivenes ...[more]

PMID: 36134940

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Dataset Information

Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface.

Publications

Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface.

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