Transcriptomic Harmonization as the Way for Suppressing Cross-Platform Bias and Batch Effect.

ABSTRACT: (1) Background: Emergence of methods interrogating gene expression at high throughput gave birth to quantitative transcriptomics, but also posed a question of inter-comparison of expression profiles obtained using different equipment and protocols and/or in different series of experiments. Addressing this issue is challenging, because all of the above variables can dramatically influence gene expression signals and, therefore, cause a plethora of peculiar features in the transcriptomic profiles. Millions of transcriptomic profiles were obtained and deposited in public databases of which the usefulness is however strongly limited due to the inter-comparison issues; (2) Methods: Dozens of methods and software packages that can be generally classified as either flexible or predefined format h

SUBMITTER: Borisov N 

PROVIDER: S-EPMC9496268 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature