Analysis of Phosphatase Activity in a Droplet-Based Microfluidic Chip.

ABSTRACT: We report analysis of phosphatase activity and inhibition on droplet-based microfluidic chips. Phosphatases are such attractive potential drug targets because abnormal phosphatase activity has been implicated in a variety of diseases including cancer, neurological disorders, diabetes, osteoporosis, and obesity. So far, several methods for assessing phosphatase activity have been reported. However, they require a large sample volume and additional chemical modifications such as fluorescent dye conjugation and nanomaterial conjugation, and are not cost-effective. In this study, we used an artificial phosphatase substrate 3-O-methylfluorescein phosphate as a fluorescent reporter and dual specificity phosphatase 22. Using these materials, the phosphatase assay was performed from approxi

SUBMITTER: Vasamsetti BMK 

PROVIDER: S-EPMC9496282 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature