Controversial Role of Transferrin in the Transport of Ruthenium Anticancer Drugs.

ABSTRACT: Ruthenium complexes are at the forefront of developments in metal-based anticancer drugs, but many questions remain open regarding their reactivity in biological media, including the role of transferrin (Tf) in their transport and cellular uptake. A well-known anticancer drug, KP1019 ((IndH)[Ru^IIICl₄(Ind)₂], where Ind = indazole) and a reference complex, [Ru^III(nta)₂]^3- (nta = nitrilotriacetato(3-)) interacted differently with human apoTf, monoFeTf, or Fe₂Tf. These reactions were studied by biolayer interferometry (BLI) measurements of Ru-Fe-Tf binding to recombinant human transferrin receptor 1 (TfR1) in conjunction with UV-vis spectroscopy and particle size analysis. Cellular Ru uptake in human hepatoma (HepG2)

SUBMITTER: Levina A 

PROVIDER: S-EPMC9496346 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature