Metal and pH-Dependent Aptamer Binding of Tetracyclines Enabling Highly Sensitive Fluorescence Sensing.

ABSTRACT: Tetracyclines are a widely used group of antibiotics, many of which are currently only used in veterinary medicine and animal husbandry due to their adverse side effects. For the detection of tetracyclines, we previously reported a DNA aptamer named OTC5 that binds to tetracycline, oxytetracycline, and doxycycline with similar K_D's of ~100 nM. Tetracyclines have an intrinsic fluorescence that is enhanced upon binding to OTC5, which can be used as a label-free and dye-free sensor. In this work, the effect of pH and metal ions on the sensor was studied. Mg²⁺ ions are required for the binding of OTC5 to its target with an optimal concentration of 2 mM. Other metal ions including Ca²⁺ and Zn²⁺ can also support aptamer binding. Although Mn

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC9496453 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature