NMR-Based Metabolomic Analysis for the Effects of Trimethylamine N-Oxide Treatment on C2C12 Myoblasts under Oxidative Stress.

ABSTRACT: The gut microbial metabolite trimethylamine N-oxide (TMAO) has received increased attention due to its close relationship with cardiovascular disease and type 2 diabetes. In previous studies, TMAO has shown both harmful and beneficial effects on various tissues, but the underlying molecular mechanisms remain to be clarified. Here, we explored the effects of TMAO treatment on H₂O₂-impaired C2C12 myoblasts, analyzed metabolic changes and identified significantly altered metabolic pathways through nuclear magnetic resonance-based (NMR-based) metabolomic profiling. The results exhibit that TMAO treatment partly alleviated the H₂O₂-induced oxidative stress damage of cells and protected C2C12 myoblasts by improving cell viability, increasing cellular t

SUBMITTER: Zou H 

PROVIDER: S-EPMC9496509 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature