The Adhesion GPCR VLGR1/ADGRV1 Regulates the Ca²⁺ Homeostasis at Mitochondria-Associated ER Membranes.

ABSTRACT: The very large G protein-coupled receptor (VLGR1, ADGRV1) is the largest member of the adhesion GPCR family. Mutations in VLGR1 have been associated with the human Usher syndrome (USH), the most common form of inherited deaf-blindness as well as childhood absence epilepsy. VLGR1 was previously found as membrane-membrane adhesion complexes and focal adhesions. Affinity proteomics revealed that in the interactome of VLGR1, molecules are enriched that are associated with both the ER and mitochondria, as well as mitochondria-associated ER membranes (MAMs), a compartment at the contact sites of both organelles. We confirmed the interaction of VLGR1 with key proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells. Immunocytochemistry by light and

SUBMITTER: Krzysko J 

PROVIDER: S-EPMC9496679 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature