Suppression of Hepatic PPARα in Primary Biliary Cholangitis Is Modulated by miR-155.

ABSTRACT:

Background

PPARα is a ligand-activated transcription factor that shows protective effects against metabolic disorders, inflammation and apoptosis. Primary biliary cholangitis and primary sclerosing cholangitis result in the intrahepatic accumulation of bile acids that leads to liver dysfunction and damage. Small, non-coding RNAs such as miR-155 and miR-21 are associated with silencing PPARα.

Methods

The expression of miR-155, miR-21 and PPARα were evaluated using real-time PCR on liver tissue, as well as on human hepatocytes (HepG2) or cholangiocytes (NHCs) following exposure to lipopolysaccharide (LPS), glycodeoxycholic acid (GCDCA), lithocholic acid (LCA) and/or ursodeoxycholic acid (UDCA).

Results

A reduction of PPARα in primary biliary cholangitis (PBC) livers was

SUBMITTER: Adamowicz M 

PROVIDER: S-EPMC9496720 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature