Nitric Oxide Trickle Drives Heme into Hemoglobin and Muscle Myoglobin.

ABSTRACT: Ever since the days of NO being proclaimed as the "molecule of the year", the molecular effects of this miracle gas on the globins have remained elusive. While its vasodilatory role in the cardiopulmonary system and the vasculature is well recognized, the molecular underpinnings of the NO-globin axis are incompletely understood. We show, by transwell co-culture of nitric oxide (NO) generating, HEK eNOS/nNOS cells, and K562 erythroid or C2C12 muscle myoblasts, that low doses of NO can effectively insert heme into hemoglobin (Hb) and myoglobin (Mb), making NO not only a vasodilator, but also a globin heme trigger. We found this process to be dependent on the NO flux, occurring at low NO doses and fading at higher doses. This NO-triggered heme insertion occurred into Hb in just 30 min in K562

SUBMITTER: Sumi MP 

PROVIDER: S-EPMC9496899 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature