LncRNA H19 Promotes Lung Adenocarcinoma Progression via Binding to Mutant p53 R175H.

ABSTRACT:

Background

Accumulating data suggest that long non-coding RNA (lncRNA) H19 and p53are closely related to the prognosis of lung cancer. This study aims to analyze the association and interaction betweenH19 and mutant p53 R175H in lung adenocarcinoma (LAC).

Methods

Mutant-type (Mt) p53 R175H was assessed by using RT-PCR in LAC cells and 100 cases of LAC tissue samples for association with H19 expression. Western blot, RNA-pull down, immunoprecipitation-Western blot and animal experiments were used to evaluate the interaction between H19 and mtp53.

Results

Mtp53 R175H and H19 were over-expressed in LAC tissues and cells, while H19 over-expression extended the p53 half-life and enhanced transcriptional activity. Combined with anti-p53, ShH19 can significantly inhibit tumo

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC9496924 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature