Identification of macrophage correlated biomarkers to predict the prognosis in patients with intrahepatic cholangiocarcinoma.

ABSTRACT:

Background

It has been shown that tumor-associated immune cells, particularly macrophages, play a fundamental role in the development and treatment response of intrahepatic cholangiocarcinoma (ICC). However, little is known about macrophages at the single cellular level of ICC patients.

Methods

ScRNA-seq from Zhang et al. was used in the present study to identify the genes differentially expressed in ICCs. Furthermore, transcriptomic data from TCGA datasets, IHC and flowcytometry from our cohort were used to confirm the findings. Kaplan-Meier and TIDE scores were also used for prognostic analysis and ICB responses.

Results

A significant number of macrophages were found in ICCs as compared to adjacent tissues. We then extracted, processed, and classified the macrophage

SUBMITTER: Xu L 

PROVIDER: S-EPMC9497456 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature