Fed-Batch Fermentation of Saccharomyces pastorianus with High Ribonucleic Acid Yield.

ABSTRACT: (1) Background: The degradation products of ribonucleic acid (RNA)are widely used in the food and pharmaceutical industry for their flavoring and nutritional enhancement functions. Yeast is the main source for commercial RNA production, and an efficient strain is the key to reducing production costs; (2) Methods: A mutant Saccharomyces pastorianus G03H8 with a high RNA yield was developed via ARTP mutagenesis and fed-batch fermentation was applied to optimize production capacity. Genome sequencing analysis was used to reveal the underlying mechanism of higher RNA production genetic differences in the preferred mutant; (3) Results: Compared with the highest RNA content of the mutant strain, G03H8 increased by 40% compared with the parental strain G03 after response surface model opti

SUBMITTER: Chen H 

PROVIDER: S-EPMC9497889 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature